Reactive Oxygen Species (ROS) in an Argon Plasma Jet Investigated with Respect to ROS Mediated Effects in Human Cells

摘要

The interdisciplinary field of plasma medicine demands a thorough investigation of the reaction pathways from the - in their reactive component output highly complex - plasma sources down to the no less complex cellular level. The work presents state of the art optical diagnostics such as two-photon absorption laser induced fluorescence spectroscopy of reactive oxygen species (ROS) on an atmospheric pressure argon plasma source. The detected ROS play a crucial role in the interaction of physical plasma with human cells. In order to investigate the effects of argon plasma jet generated ROS on human cells two different cell lines are employed. Adherent keratinocytes (HaCaT) are compared to non-adherent T-lymphocytes (Jurkat) with regard to proliferation, apoptosis and cell cycle changes.