EVALUATION OF THE CORNEA CELLS AFFECTED BY MULTI-PURPOSE SOLUTIONS FOR CONTACT-LENS

摘要

We studied the influence of multi-purpose solutions (MPS) on gap-junctional intercellular communication (GJIC) of cornea cells and its cell toxicity such as cell metabolism and proliferation. This study evaluates long-term safety of three types of multi-purpose solutions (MPS) in the cornea cells. Three types of MPS were investigated using scrape-loading dye transfer (SLOT) method to measure GJIC. Cell toxicity was evaluated for cell viability according to colony method and USP elution method (MEM Elution Test). In the SLDT method, one type solution was significantly showed an inhibitory effect on the GJIC in cornea cells but not the other type solutions. Comparative cytotoxicity potentials of three types of MPS also indicated similar tendency in the result of SLDT method. These results suggest that the evaluation methods are considered to be effective to confirm the safety of MPS.