Effects of the incorporation of proteins and active enzymes on biomimetic calcium-phosphate coatings

摘要

In order to investigate the influence of biomolecules on the formation of a calcium phosphate (Ca-P) layer on a starch based biodegradable polymer, proteins and enzymes were incorporated on Ca-P biomimetic coatings. In this study, a biodegradable blend of corn starch with cellulose acetate (SCA) was used as substrate and, by means of adapting a biomimetic route, the formation of a bioactive layer was evaluated. Bioactive glass (45S5 Bioglass) was used as nucleating agent. Two different types of conditions were studied: (i) the addition of a-amylase or bovine serum albumin (BSA) to a simulated body fluid (SBF) solution and (ii) the addition of α-amylase or BSA to the bioactive glass used to nucleate the coating. The obtained results indicated that both proteins and enzymes could significantly change the morphology and growth of biomimetic Ca-P coatings. Furthermore, this work clearly showed that the incorporation of active enzymes allows for controlling the degradation rate of starch-based polymeric materials.