Characterization of cDNA sequences differentially expressed in wheat (Triticum aestivum L.) spikelets

摘要

In the present research 85 cDNA sequences specifically .expressed in wheat spikelets at heading time have been identified and cloned by a method of differential analysis of transcripts (SDDM). The search of their nucleotide and deduced amino acid sequences in several databases allowed their assignment, on the basis of their putative functions, to four main functional groups: i) sequences involved in the morphogenesis of floral organs; ii) sequences expressed in anther tissues and/or pollen; iii) transcription factors; iv) kinases and LRR proteins. Some sequences were also cloned that are probably related either to the intense cell proliferation which takes places within the floral organs, such as cyclins, microtubule and centromeric proteins, or inthe protein trafficking within the secretory pathway. The expression patterns of several cloned sequences have been studied by RT-PCR analysis.