小麦(Triticum aestivum)根系在不同钾营养水平条件下的蛋白质组差异表达分析

摘要

[Objective] This study aimed to investigate the regulatory role of potassium levels in plant gene expression. [Method] By using wheat (Triticum aestivum L. Beinong 9549) as experimental material, the protein expression of wheat roots under different levels of potassium was determined by two-dimensional electrophoresis (2-DE). [Result] Compared with the plants supplied with normal level of potassium, 152 protein spots in wheat roots under potassium deficiency condition showed significant differences (P < 0.05), including 76 protein spots significantly up-regulated by more than two times and 76 protein spots significantly down-regulated by more than two times. After potassium deficiency treatment, the wheat plants were supplied with normal level of potassium for two weeks, which showed significant differences in 73 protein spots compared with the plants continuously supplied with normal level of potassium (P<0. 05) , including 36 protein spots significantly up-regulated by more than two times and 37 protein spots significantly down-regulated by more than two times. [Conclusion] This study indicates that potassium deficiency stress would lead to induction of a large number of specific proteins in wheat roots.%[目的]了解不同小麦根际钾元素水平对其根系基因表达的调控.[方法]采用小麦(北农9549)为试验材料,在缺钾胁迫和胁迫后恢复钾供应等不同条件下,用双向电泳的方法检测不同钾营养水平条件对根系蛋白质表达的调控.[结果]与正常供钾的植株相比,缺钾条件下生长的小麦根系有152个蛋白点的表达水平变化具有显著差异(P＜0.05),其中76个蛋白点发生大于2倍的显著上调表达,另有76个蛋白点发生大于2倍的显著下调表达；经过钾饥饿处理的小麦根系恢复正常钾供应2周后,与一直正常供钾的根系相比,有73个蛋白点的表达水平变化具有显著差异(P＜0.05),其中36个蛋白点发生大于2倍的显著上调表达,另有37个蛋白点发生大于2倍的显著下调表达.[结论]该试验结果表明缺钾胁迫可以引起小麦根系表达大量特异性蛋白质.