ENHANCED BROWNIAN RATCHET ARRAY FOR DNA SEPARATION USING FLOW ANGLE EFFECT

摘要

Under strict control of ion flows, we examine the effect of the flow direction with respect to the array on the separation efficiency. Devices for flow angles of 0°, 4°, 8° and 12° are made. The separation angle between bands of 48.5 kb and 164 kb DNA molecules is maximized around 8°, for the specific array design. The enhanced performance of the Brownian ratchet array implies that the array could potentially be a useful device for DNA separation in the ～100 kb range.