MICRO ISOELECTRIC FREE FLOW SEPARATION OF SUBCELLULAR MATERIALS

摘要

We report a microfabricated field flow fractionation device for continuous rapid separation and concentration of subcellular organelles (e.g. nuclei, peroxisomes, and mitochondria) by free-flow isoelectric focusing (IEF) from crude cell lysate. We show that different organelles or organelles of different physiological states can also be separated using this technique. The collection of different fractions is accomplished by incorporating end channels, taking advantages of the laminar nature of the flow field.