Detection of swine influenza virus (SIV) in porcine oral fluid samples

摘要

In humans, oral fluids are used for viral detection, antibody detection, drug monitoring (drug abuse and therapeutic drug monitoring), and to monitor hormone levels. In pigs, oral fluids have recently been used as a surveillance tool for porcine reproductive and respiratory syndrome virus (PRRSv) and PRRSv antibodies using quantitative reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay and indirect fluorescent antibody tests. This method used a 3-strand twisted cottonrope (5/8 inch diameter) that was suspended within a pen of 4 to 6 pigs. The pigs chewed on the rope, moistening it with oral fluids, and then the oral fluids were collected for analysis.The objective of this present study was to determine whether swineinfluenza virus was present in oral fluids at diagnostic levels.