Use of an experimental model to test the efficacy of planned exposure to live porcine reproductive and respiratory syndrome virus (PRRSV)

摘要

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be an economically devastating disease.' Commercially available PRRSV vaccines fail to effectively control PRRS on some farms and this has led some veterinarians to implement theuse of planned exposure to live PRRSV as a means to induce protective immunity in growing pigs and/or breeding age animals prior to natural exposure. Detailed descriptions of techniques for preparation of the serum injection, the administration of the inoculum, the safety of the procedure and anticipated efficacy are lacking in the literature. The objectives of this study were to establish a detailed protocol, evaluate the amount of PRRSV needed in donor serum to immunize naive animals, and determine the efficacy of this procedure to provide homologous and heterologous immunity.