RETROSPECTIVE IMMUNOHISTOCHEMICAL STUDY ON PORCINE CIRCOVIRUS TYPE 2 (PCV-2) IN THAILAND

摘要

Porcine circovirus (PCV)-2, a small, single-stranded, circular DNA virus, is a cause of postweaning multisystemic wasting syndrome (PMWS) in pigs. Currently, the disease has been reported worldwide. In Thailand, we previously reported the detection ofPCV-2 in weaning pigs using electron microscopy and the nested PCR from the formalin-fixed, paraffin-embedded (FFPE) tissues. The immunohistochemical (IHC) detection of PCV-2 antigen from pig tissues has been reported to be sensitive, reliable and inexpensive compared with in situ hybridization. However, the appropriated tissues of pigs for routine diagnosis have not been clarified. The purpose of this work is to study the distribution of PCV-2 antigen in PMWS pigs in Thailand using the IHC method.