A RECOMBIANT NUCLEOPROTEIN-BASED ELISA FOR DETECTION OF ANTIBODY AGAINST PRRS VIRUS

摘要

Porcine Reproductive and Respiratory Syndrome (PRRS) has been regarded as an economically important swine disease in many swine-producing countries since it was first reported in America in 1987 (2). PRRS virus causes respiratory problem, reproductivefailure in pregnant sows and deaths in piglets. PRRS has been spread throughout most of provinces in China after it broke out first in China 1996 (1). Now many methods for the detection of PRRSV antibodies have been established, such as IMP A, ELISA andIFA. And ELISA is high-throughput method for PRRS diagnosis and hematological investigation. Because the antigen of PRRSV-infected cell lysates has some shortcomings,improved test strategies are needed. The objective of this study was to develop a rapid, inexpensive alternative method for detecting antibodies against PRRSV, using recombinant PRRSV Nucleocapsid protein.