Antigen capture anti-dengue IgA ELISA for the detection of flavivirus specific antibodies (ACA-ELISA)

摘要

We have developed antigen capture IgA Enzyme Immunoassay for the detection of anti-flavivirus IgA and (ACA-ELISA). The assay, flavivirus dissolved antigen, preferably using a dengue virus lysis antigen captured by the monoclonal antibody. Anti flavivirus IgA captured from the test serum is detected preferably a reporter group, for example using labeled rabbit anti-IgA horseradish peroxidase (HRP). The assay has been found to be at least 8 times more sensitive than the anti-human IgA capture ELISA (AAC-ELISA). ACA-ELISA based on either serum or saliva, it has been found to be a rapid at higher sensitivity compared with the "Golden Rule" anti-dengue IgM detection techniques. This can be used as a diagnostic tool for dengue confirmation in early infection.