BACTERIAL STRAINS FROM MOLDY BUILDING ARE HIGHLY POTENT INDUCERS OF INFLAMMATORY AND CYTOTOXIC EFFECTS

摘要

Inflammatory responses and cytotoxicity induced by bacteria, isolated from moldy buildings, were evaluated in human and mouse cells and in a mouse. Human (28SC)- and mouse (RAW264.7) macrophage cell lines were exposed to the spores of Streptomyces californicus and bacterial cells of Mycobacteriun terrae at different doses in vitro and mice (NIH/S) were exposed by intratracheal instillation to the same microbes. The results show that S. californicus and M. terrae induce production of important inflammatory mediators i.e. nitric oxide (NO) and cytokines both in human and mouse macrophages in vitro, but mouse cells were more sensitive than human cells. In line with in vitro findings, these bacterial strains triggered in vivo time dependent production of the same inflammatory mediators and caused cytotoxicity in mouse lungs. Altogether, these results suggests that in indoor air streptomycetes and mycobacteria are potent inducers of inflammatory responses possibly related to adverse health effects of the inhabitants.