HIGH-EFFICIENCY SEPARATION IN MICROFLUIDIC DEVICES FOR HIGH-THROUGHPUT SCREENING OF KINASES

摘要

A new approach for performing on-chip mobility shift assay on a LabChip device has been developed. The separation of PKA product and substrate was conducted on a microfluidic chip using a multi-port pressure/voltage controller. The product is selectively extracted from the flowing mixture and detected downstream by controlling the pressure and voltage at the two waste wells. This approach significantly reduces the background fluorescence signal and permits detection of conversions as low as a few percent, thus increasing the number of kinases that can be assayed.