COMBINED NUCLEIC ACID EXTRACTION AND ENRICHMENT IN BEAD-PACKED PLASTIC BEDS

摘要

Sample pre-treatment and DNA analysis are recent trends in microfluidics. In this paper, we describe nucleic acid extraction from a poly(dimethylsiloxane) (PDMS) channel packed with silica beads. The extraction of nucleic acids from biological samples such as Human Papilloma Virus (HPV) and human cell lysates were successful, with the collection of the nucleic acids in the first 10 μL of eluate. The integration of platinum electrodes just below the packing is being explored as way to reversibly collect the eluted nucleic acids and preconcentrate them on-chip.