Objective The active ingredient was used as index to optimize the extraction and enrichment process of anti-in-flammatory and analgesic active-fraction(ARF)of Dianbaizhu. Methods Methyl salicylate triglycoside-B was chosen as index com-ponent to extract and enrich methyl salicylate glycosides. Extraction and elution solvents were optimized. The HPLC fingerprint was ob-tained with Thermo Hypersil Gold C18(250 mm×4.6 mm,5μm)column and a gradient elution with the mobile phase consisting of ace-tonitrile(A)-0.2％acetic acid(B)at a flow rate of 1.0 ml/min. And the detection wavelength was set at 294 nm. Results The opti-mized extraction solvent of Dianbaizhu was the 30％ethanol and the optimized elution solvent of ARF enriched by AB-8 macroporous resins was the 35％ethanol. The methodological study on similarity and RSD in ARF HPLC fingerprint of three batches of samples cor-responded to related regulations. Conclusion The extraction and enrichment process of ARF is stable and repeatable.%目的 以活性成分为指标,优化滇白珠抗炎镇痛活性部位(ARF)提取富集工艺.方法 以水杨酸甲酯三糖苷(MSTG-B)为指示性成分对滇白珠水杨酸甲酯糖苷类成分进行提取及富集,确定最佳提取和洗脱溶剂.选用Thermo Hypersil Gold C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.2％乙酸溶液为流动相进行梯度洗脱,流速为1.0 ml/min,检测波长为294 nm,建立滇白珠ARF部位的HPLC指纹图谱.结果 滇白珠药材的最佳提取溶剂为30％乙醇溶液,经大孔树脂AB-8富集后的35％乙醇洗脱部位为ARF部位.所测定的3个不同产地的滇白珠样品,其ARF部位的指纹图谱相似度和方法学考察均符合要求.结论 ARF部位提取富集方法工艺稳定,重现性好.
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