Amounts and Concentration of Vascular Endothelial Growth Factor in GCF

摘要

The aim of the present study was to monitor the changing levels of vascular endothelial growth factor VEGF in gingival crevicular fluid GCF following scaling and root planing SRP, for two months. Twenty-two sites from 11 patients with moderate to severe periodontitis were subjected to the study. Clinical parameters such as probing attachment level PAL, probing pocket depth PPD, plaque index PI, gingival index GI, and bleeding on probing BOP were recorded at baseline prior to SRP, 2 weeks, 1 and 2 months after SRP. GCF samples were collected at each site and volume determined using Periotron 8000. VEGF was assessed with using the enzyme-linked immunosorbent assay ELISA. 22 sites were classified into three groups according to i the pocket depth PD>5 mm, 35 mm, 35 mm group than 35 mm group than in the PAL<3 mm. The concentration of VEGF did not differ between the groups. Thus the baseline data demonstrated that the total amounts of VEGF were related to probing pocket depth and probing attachment level, but the concentrations were not. The sites which bled on probing at the baseline received SRP and were classified into two groups according to the presence or absence of BOP measured at 2 months i BOP + group 8 sites, ii BOP - group 6 sites. There was no significant difference in any clinical parameters at baseline between both groups. The mean PPD and GI revealed a significant decrease in both groups. The mean PPD and PAL did not differ significantly at 2 months between two site groups. There were no significant differences in the total amount of VEGF at any periods for 2 months between the two groups. The concentration of VEGF in GCF was lower at 2 weeks in the BOP - sites than in the BOP + sites. These results obtained at sites treated with SRP suggest that the concentration of VEGF in GCF at 2 weeks might have predicted the healing response of periodontal tissues following SRP.