Enzyme-substrate specific interactions: in situ assessments under pressure

摘要

Peroxidase (POD) enzyme was irreversibly inactivated (>90%) after 2 minutes at 500 MPa/25°C in the presence of guaiacol. POD reaction with ferulic acid or with catechol monoethyl ether as substrates is not influenced by pressure up to 500MPa (25°C). POD reaction with guaiacol or with vanillin as substrates is strongly inhibited (reversibly) by pressures above 300MPa (25°C). Polyphenoloxidase (PPO) reaction with chlorogenic and caffeic acid as substrates is not influenced by pressure up to 500MPa (25°C). PPO reaction with pyrocatechol as substrate is strongly inhibited (reversibly) by pressures above 300MPa (25 and 40°C).