The protective effect of an osmotic perturbation on high-pressure inactivation has been investigated using a yeast strain: Saccharomyces cerevisiae. Osmotic shocks have been carried out with binary medium (glycerol/sorbital and water) with or without glucose. Variations of osmotic level, osmotic shift kinetics and time between osmotic and hydrostatic treatment (350 MPa/10 min) have allowed the characterization of cell baroprotection. Two different cumulative baroprotection effects have been distinguished: one conferrec by the solute on biological structures and the other related to the osmotic stress, cell volume variations and stress metabolism response (HSP, trehalose). These experiments have also exhibited a repair phase, which occurred immediately after the high-pressure treatment and required the presence of glucose.
展开▼
