A DIP-STICK ASSAY FOR DEXTRAN ANALYSIS

摘要

With the goal of developing a rapid, inexpensive method for measuring dextran concentrations in cane juice, we selected a dextran specific phage that can be used as an assay reagent. Phage antibody libraries express the universe of antigen binding fragments (Fab) as protein ligands. After serial panning, monoclonal phages for dextran were chosen, using a Sephadex agarose electrophoresis system (SAES). Dextran binding affinity was determined and a paper dip-stick assay was constructed. This assay, when tested on core lab juice, showed good correlation with the Midland Antibody test for dextran. It is a method that is both quick enough and cheap enough for potential use in screening for dextran in loads of cane arriving at the sugar mill.