3-D Bone Tissue Engineering with Bioactive, Resorbable Microcarriers

摘要

Novel bioactive, degradable polymer/bioceramic composite microspheres were developed using a solid-in-oil-in-water (s/o/w) emulsion solvent removal method. Modified bioactive glass (MBG) powders were encapsulated into the polylactic acid (PLA) matrix. The microsphere surfaces were transformed into bioactve bone apatite via immersion in simulated physiological fluid (SPF). Osteoblastic cell cultures with the microspheres showed that the cells attached to and formed 3-D aggregates with the microspheres. Extensive extracellular matrix formation and mineralization were observed in the aggregates. Our results support the concept that these new bioactive, degradable composite microspheres may serve as microcarriers for synthesis of bone and other tissues in vitro and in vivo.