Simultaneous analysis of variable and delayed chlorophyll fluorescence during induction period in photosynthetic apparatus

摘要

Luminescent methods based on registration of emitted by the photosynthetic apparatus quanta of prompt and delayed chlorophyll fluorescence (PF and DF) are one of most perspective biophysical methods for express analysis of the plant state. Although light quanta are emitted by the same population of molecules, the two types of fluorescence contain information about different fundamental processes of the photosynthetic apparatus (Vesselovski and Vesselova 1990; Krause and Weis 1991). The simultaneousregistration and analysis of both luminescence types enriches our knowledge and understanding about the current state of the investigated object. In the present work we describe equipment for synchronized registration of PF and DF induction kinetic curves (IKC) in the micro- and millisecond time range, as well as for analysis of photoinduced changes in the kinetics of delayed fluorescence dark relaxation.