Capillary electrophoretic enzyme immunoassay with electrochemical detection was set up. The method has advantages of both enzyme immunoassay and capillary electrophoresis. In the assay an on-line enzyme catalysis system was designed. There was a metal needle of syringe in a homemade plexiglas vessel. Both the fused-silica separation capillary and the reaction capillary were inserted in the needle with a very narrow gap between them.
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