Genetic analysis of temperature-sensitive genie male sterility in rice

摘要

Crosses were made between two TGMS lines, 1D24 X SM5 and IC10 X jPs4. The F_1s had completely normal pollen and spikelet fertility under high temperature (35 deg C day/26 deg C night). The F_2s showed a 9 fertile:7 sterile ratio, indicating that the character is governed by two separate genes. Pollen sterility in the F_2 ranged from completely sterile to 27% sterility, indicating the effect of modifier genes under high-temperature regimes (during April 1998 at Tiruchirapalli, India). Testcrosses madebetween TGMS and non-TGMS lines were used to determine the F_2 segregation pattern under higher temperature. The eight most promising F_1S based on their yield performance were selected and F_2 segregation under high temperature was carried out for pollen fertility. The F_2 segregated into a 3 fertile:1sterile (TGMS) ratio under high temperature (>35.5 deg C day and >25.6 deg C night), indicating that the TGMS trait is governed by a single gene.