Genetic Analysis and Molecular Mapping of Gene Associated with Dominant Genie Male Sterility in Rapeseed (Bmssica napus L)

摘要

Rsl046AB is a dominant genie male sterility (DGMS) line derived from a spontaneous mutant in Brassica napus. The sterihty of this mutant was previously regarded as to be eonditioned by the interaetion of a dominant male sterihty gene Ms and its non-allelie dominant restorer geneMf(or Rf in previous reports). Recent genetic analyses, however, indicated that Ms and Mf may be alleic In this research, we confirmed that a multiple allele model should be more appropriate to elucidate the heredity of DGMSline Rs1046AB. Based on this result, the present study emphasized on identifying DNA markers linked to Ms/Mf in an F_2 population constructed by crossing Rsl046A with a double haploid (DH) restorer hne (19514A). Twenty ampHfied fragment length polymorphism (AFLP) markers Unked to the Ms/Mflocus were identified by combination of bulked segregant analysis (BSA) with AFLP technique. The target locus was detected to be co-segregating with the marker S05T05 and bracketed by two nearest markers, E14M01 and E01M02, with a genetic distance of 0.1 cM and 1.2 cM, respectively. Furthermore, we successfully converted two AFLP markers into sequence characterized amphfied region (SCAR) markers. These findings provided direct molecular marker proofs on the inheritance model of this DGMS hne, and the high density molecular markers hnkage map aroimd the Ms/Mflocus will be more informative for both marker-assisted selection (MAS) of elite male sterile lines and isolation of the Ms/Mf genes by positional cloning in future.