Doubly dye labeled peptides that utilize FRET quenching have many applications.A new class of efficient quencher molecules,termed Black Hole Quenchers~(TM),have been introduced into peptides via phosphoramidite derivatives coupled to the hydroxylic functions of serine,tyrosine,or hydroxyproline residues.The spectral characteristics for the entire family (BHQ-1,BHQ-2,etc) are matched to sets of fluorophores,providing high sensitivity and low background detection in a variety of fluorogenic assays.
展开▼