Analysis Of DNA-Polyamine Interactions Using SERS-Coated Microplates In A Dedicated Raman Plate Reader

摘要

Polyamines are well known to play a significant role in cell functioning [1-6]. All known cellular organisms possess polyamines such as spermine, putrescine, and others, often in significant (up to 10mM), but always tightly regulated, concentrations [7]. Although the precise role of polyamines in cellular processes is not always understood, some important areas of polyamines' involvement are known. These include regulation of levels of c-fos, c-jun and other transcriptional factors [1] and participation in early signal transduction and cell proliferation [2], control of growth arrest and apoptosis [3], sequence-specific binding to DNA and regulation of chromatin acetylation [4, 5].