Simultaneous Expression of Vitreoscilla Hemoglobin gene and Lytic genes of Phage in the Novel Recombinant Escherichia coli Producing PHB

摘要

Heterogeneous Vitreoscilla hemoglobin gene (vgb),lytic genes of phage A with S amber mutation (S-RRz) and PHB biosynthetic genes (PhbCAB) were cloned into the same Escherichia coli host,simultaneously or respectively,and some novel recombinant strains were constructed.The novel strain VGl (pTU14),in which the genes vgb,phbCAB and S-RRz could all be successfully expressed,had superior characteristics in the cell growth and PHB accumulation.While the results of the strains harbored the vgb and phbCAB genes without S-RRz,do not better than that of the strains harbored the phbCAB only.The simultaneous expression of vgb and S-RRz in the recombinant E.coli producing PHB presented the great potential for the low-cost industrial production of PHB.