Caspases as a target for apoptosis inhibition in hybridoma cells in culture

摘要

he importance of glutamine as the major apoptotic inductor in batch cultures of an hybridoma cell line has been investigated.The importance of the caspase cascade, as a possible target for apoptosis inhibition, in the signaling of apoptosis in cultures deprived of glutamine has been also studied. The specific peptidic inhibitor of caspases Ac-DEVD-CHO partially inhibited (25%) the apoptotic process while cysteine protease inhibitor Z-VAD-FMK was a much more effective inhibitor of apoptosis (75%).On the other hand, only with the combination of both inhibitors has been possible to make reversible the programed cell death process.