Microbiological determination of sulfadimethoxine and oxytetracycline residues followed by liquid chromatographic identification in swine muscle

摘要

Oxytetracycline and sulfadimethoxine are widely used for treatment of bacterial diseases in food-producing animals and poultry. However, the use of these products may lead to the presence of residues of the parent drugs or their metabolites in foodand animal tissues. Many authors have published various methods for the determination of oxytetracycline and sulfadimethoxine in serum, milk, tissues, premixes, and feeds. In general, the inspection of residual antimicrobial agents in meat is performed according to the following steps: 1) detection by microbiological assay, 2) extraction (clean-up) from the residual-positive, and 3) identification by HPLC using its extract. In this study, we detected oxytetracycline and sulfadimethoxine residues using microbiological method following by liquid chromatographic identification in swine muscle. Test organisms and assay media. Mueller-Hinton agar (MHA, Difco) and minimum medium (MM) were used as assay media for Bacillus (B.) subtilis. The other test organisms and assay media used were as follows: Micrococcus luteus ATCC-9341 (M. luteux) with MHA, Bacillus cereus var. mycoides ATCC 11778 (B. cereus) with MHA.