Experimental in vitro study of the dissolution of bioactive glass (S53P4) in amounts used in the obliteration the larger bone cavities

摘要

Bioactive Glass S53P4 (BG) is an osteoconductive allograft material used in obliterating larger bone cavities e.g. frontal sinuses. In vitro model was used to investigate the behaviour of a massive frontal sinus obliteration with BG to estimate the resorption of BG at the obliterated cavities. Two sizes of granules in sixteen separate BG amounts, weight 25 g, were tested both in simulated body fluid (SBF) and a buffer containing trishydroxymethyl aminomethane citric acid (TRIS-c.a) in standard conditions. The dissolution of silicon (Si) and phosphate was detected with current plasma atom emission spectroscopy (DCP-AES) monthly up to 6 months. The calcium phosphate (CaP)-and silica (Si)-gel-layers were studied by scanning electron microscopy (SEM) at 1, 3 and 6 months. The cumulative loss of Si and P was stronger in TRIS-c.a than in SBF (p<0.0001) and it was higher with smaller than larger granules in both solutions (p<0.0001). In SBF soaked BG amounts, the CaP-layer occurred in the upper part of BG amount on the uppermost granules. In TRIS-c.a, at 3 to 6 months, CaP-layer occured on the granules in the centre and lower parts of BG amount. BG seems to be a durable filling material for frontal sinuses.