A study of the effect of PTFE electret on fibroblast cell cycle with flow cytometry(FCM)

摘要

In this paper, the PTFE films were respectively charged at ± 300V, ±500V and ±1000V by corona charge system. After applied on the cells for 24h, 48h and 72h, the influence of electret on fibroblast (3T3 cell) cell cycle was studied with flowcytometry. The results indicated that 1) after action of negative PTFE electret field for 24h, the cell number in G{sub}1 phrase and G{sub}2 phrase decreased, but increased in S phrase. It suggested that negative electret can improve cell's growth. 2)negative electret can not only enhance the growth of cells, but also enhance the apoptosis of mutant cell. 3) after action of positive electret field on fibroblast for 24h, the cell number in G{sub}1 phrase increased, while the cell number decreased in Sand G{sub}2 phrase. Positive electret can block some cell in G{sub}1 phrase. So it can restrain the growth of fibroblast cell. In short, electret has bilateral regulation effect on the enhancement and block of cell growth.