Tissue engineering of goat bone: osteogenic potential of goat bone marrow cells

摘要

Goat bone narrow cells were isolated from the iliac crest of adult Dutch milk goats and grown under culture conditions facilitating osteogenic differentiation. The culture medium comprised of alpha -MEM supplemented with 15percent foetal bovine serum and with or without dexamethasone, L-ascorbic acid-2-phosphate and beta -glycerophosphate. The in vitro osteogenic potential of the cells was examined by the production of a bone-like mineralised extracellular matrix. After seeding and culturing the cells for 1 week in porous calcium phosphate particles followed by subcutaneous implantation in nude mice for 4 weeks, the in vivo osteogenicity of these hybrid samples was examined by determining de novo bone formation.