METABOLIC RESPONSES OF EUKARYOTIC DRUG EFFICACY AND TOXICITY

摘要

Identifying metabolic responses of cells to drugs can be highly informative for understanding mode of action and toxicity, and can provide value in the drug discovery process by complementing conventional pharmacokenetic/pharmacodynamic measurements.In many circumstances, drugs are known to impinge upon biochemical pathways comprising previously identified intermediates, of which there may be a necessity to assay during development. To address these concerns in the pharmaceutical industry, we have engineered a targeted analytical platform that can rapidly assay a predefined panel of metabolites. We are applying the technology to investigate the responses of predefined metabolites to several classical drugs in a eukaryotic cell model system. A simple and intuitive interface allows users to quickly associate aberrantly regulated pathways with specific drug activity.