Action of low-energy monochromatic coherent light on the stability of retinal lysosomes

摘要

The data had been obtained during the experiment in vitro by irradiation of solubilized lysosomal enzymes, retinal homogenates and native lysosomes enabled us to conclude that the laser beam ($lambda $EQ 632.8 nm, power density from 0.1 to 15.0 mWt/cm$+2$/) acts on the level of membranous structures of lysosomes. During irradiation of rabbits eyes in vitro with an unfocused laser beam (power density on the cornea aur face from 0.01 to 15.0 mWt/cm$+2$/ was shown, that low-energy, ranged from 0.01 to 1.0 mWt/cm$+2$/ promotes stabilization of lysosomal membranes. Irradiation with laser beam of 8.0 mWt/cm$+2$/ and more power induces destabilization of lysosomal membranes. We have also shown that vitamins A and E effecting membranotropic on lysosomes may be corrected by low-energy radiation of helium-neon laser. It is substantiated experimentally that the stabilizing effect of vitamin E may be intensified in case of the combined action of laser radiation on lysosomes. The labilizing effect of vitamin A on membranes of organelles, as was studied, may be weakened by application of laser radiation of low intensities.