Insight into the plasma membrane resealing and calcium signaling dynamics of sonoporation

摘要

Ultrasound (US) and microbubble (MB) treatment has been shown to be a promising approach for localized, non-viral gene delivery. The attributes of MB-target cell interactions that facilitate payload delivery across cell membranes and into extravascular cells, and hence strategies to optimize this platform, remain poorly understood. The objective of this work is to gain insight into the extent of sonoporation by assessing cell membrane dynamics and Ca2+ signaling, both known factors in cell perforation repair, and the role of gap junctional communication (GPC).