Sequestration of Reactive Light-Yellow K-4G by free Mycobacterium phlei cells and its extracellular polysaccharide

摘要

Production of extracellular polysaccharide by the culture of Mycobacterium phlei were examined,using molasses wastewater to replace glucose as carbon source and energy source in the culture medium.Results showed that the COD concentration in molasses wastewater favorable for the growth of the Mycobacterium phlei cell was 5000 mg·L-1,and inoculum size of 5％(v/v),28°C,initial pH 7.0 and shaking speed of 150 r·min-1,under the optimal culture conditions,the highest flocculating activity achieved for Kaolin suspension was 95.5％ and 5.0 g cell/L broth was obtained.The Mycohacterium phlei cell was effective in flocculating some soluble reactive dye in aqueous solution,reactive Light-Yellow K-4G with a decolorization efficiency of 93.5％,respectively,using 20 mL of the flocculant in 500 mL of 100 mg·L-1 dye solution.