Size and Count of Nanoparticles by Scattering and Fluorescence Nanoparticle Tracking Analysis (NTA)

摘要

A novel addition to a technique for the analysis of nanoparticles in a suspension is described. The Nanoparticle Tracking Analysis (NTA) technique sizes individual nanoparticles, based on their Brownian motion. NTA allows nanoparticles to be sized on a particle-by-particle basis, resulting in a higher resolution analysis and therefore a better understanding of polydispersity than ensemble methods (such as dynamic light scattering, DLS) and it also yields directly a count/concentration measurement. Analysis of scattering intensity is a recent development allowing sub-populations of nanoparticles with varying scattering characteristics to be resolved in a complex mixture.Now this technique has been extended to the analysis and differentiation of fluorescently labeled nanoparticles. With the appropriate wavelength lasers and optical filters, the technique has been shown to be able to differentiate between sub-populations in a heterogeneous mixture.