Cellular Biological Influences of Down-Regulated MicroRNA-143 on Human Bronchial Epithelial Cells

摘要

MicroRNAs (miRNAs) were recently identified to play crucial roles in tumorigenesis processes. In present study, we explored the potential influence of miRNA-143 on several cellular biological processes related with lung cancer development in human bronchial epithelial cells 16-HBE. Chemosynthesis anti-miR-143 was used to down-regulate expression of miR-143. Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay after 24, 48 and 72 h transfection of anti-miR-143. Apoptosis and cell cycle were detected by flow cytometry after 48 h transfection of anti-miR-143. The results revealed that there were no significant differences in cell activity and apoptosis between transfection group and control group (P>0.05). Compared with control group, DNA content of G1 period of anti-miRNA-143 group had a mild decrease (P＜0.05), DNA content of S period of anti-miRNA-143 group had a mild increase (P＜0.05). The results suggested that down-regulated miR-143 induce S arrest of cell cycle. MiR-143 may associate with lung cancer development through influence cell cycle progression. The cooperation between more miRNAs and environmental carcinogen on the tumorigenesis of lung cancer needs to further investigate.