Minocycline nano-liposome inhibit the production of TNF-α in LPS-stimulated macrophages

摘要

To evaluate the effects of minocycline nano-liposome on regulating endotoxin-induced upregulation of inflammatory molecules were elucidated. Murine macrophages (ANA-1 cells) were prepared and treated with lipopolysaccharide (LPS, 10 ¿g/ ml), LPS plus minocycline nano-liposome (10, 20, 40, 50 and 70 ¿g/ ml), LPS plus minocycline (50 ¿g/ ml). MTT assay was used to evaluate the in vitro cytoxicity. The levels of TNF-¿ mRNA were measured by fluorescent RT-PCR. Minocycline nano-liposome showed dose- and ratio-dependent inhibition. LPS-stimulated inflammatory cytokine TNF-¿ secretion in the macrophages and reduced LPS-induced TNF-¿ mRNA expression on macrophages. It shows statistically significance when compared minocycline nano-liposome (10, 20, 40, 50 and 70 ¿g/ ml) to the vehicle control group; Statistic difference (P>0.05) was not found between minocycline nano-liposome (10, 20, 40 ¿g/ ml) and minocycline (50 ¿g/ ml). It indicated that the minocycline nano-liposome can provide a potential therapeutic tool to reduce the amount of drug that is required to treat a inflammation or immune diseases of periodontitis associated with the production of TNF-a, and have less side effect.