Aspergillus niser for the study of in vitro drug metabolism

摘要

Drug metabolism is an important aspect for the study of toxic effect in human being. However, the occurrence of several regulatory issues and high cost involved in experimental study on drug metabolism, restrict the study using human as a model system. Keeping this in view, in the present study, the fungus Aspergillus niser was chosen as a model organism for the study of Clemastine metabolism as it is cheap and expresses several mammalian enzymes. The metabolism study was performed using Aspergillus niser and human and a comparative study has been done. The experimental result has established that Aspergillus niser can be a potential organism for drug metabolism study. Further, HPLC was found to be an effective analytical tool for the characterization of metabolites produced by the enzymatic reaction.