PROTECTION OF ISORHAMNETIN AND LUTEOLIN AGAINST SIMULATED MICROGRAVITY INDUCED OXIDATIVE STRESS IN SH-SY5Y CELLS

摘要

Spaceflight is known to produce a number of neurological disturbances which are of unknown etiology. A variety of evidences suggest that simulated microgravity results in increased oxidative stress in nervous system. Therefore, it is becoming important to supplement some effective antioxidants to inhibit the increased oxidative stress induced by space flight or microgravity in order to ensure the safety of prolonged space travel. In the present study, using clinostat to simulate microgravity, we found that the level of reactive oxygen species (ROS) was increased time dependently, and confocal images also demonstrated time-dependant disassembly of actin microfilament concomitantly with the change of ROS in neuronal SH-SY5Y cell after clinorotation. Flavnoids such as isorhamnetin and luteolin treatment protected neuronal cells by preventing from microgravity induced increase in ROS. Furthermore, isorhamnetin and luteolin could partially prevent the disassembly of cytoskeleton during the clinorotation. These results indicated that the disorganization of cytoskeleton under simulated microgravity condition might be partly related with oxidative stress, the isorhamnetin and luteolin could protect against microgravity induced oxidative stress and cytoskeleton disorder.