A sensitive solid-phase time-resolved fluorescence immunoassay apparatus

摘要

In the device, a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source, and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA). The first one is to design optimum exciting optical system, so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least, the sample fluorescence through the integrating sphere and come to the grating monochromator, The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device, SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process,signal-to-noise ratio(SNR) will be improved ,also adjust delay time、 sampling frequency and sampling number arbitrarily. By testing, the sensitivity is 10~(-12) mol/L(substance marked by Eu~(3+)), examination repeat is 22.5%, examination linearityis from 10~(-8)mol/L to 10~(-12)mol/L , correlation coefficient is 99.98%(p≤0.01). The instrument is advanced for ultrasensitivedetection of antigen and antibody , and solve the tumor, genetic variation, the virus protein detection.