ELECTROSPUN POLYMER NANOFIBERS PACKED MICRO-COLUMN FOR THE SOLID-PHASE EXTRACTION OF PAPAVERINE IN HUMAN PLASMA

摘要

A novel microextraction procedure based on micro-column packed with polystyrene electrospun nanofibers as the sorbents was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC-UV) for analysis of papaverine in human plasma. In the extraction procedure, the plasma sample was loaded onto the micro-column, and pushed through the column bed by a gas tight plastic syringe. The targets were absorbed onto the nanofibers. After washing sorbent with water, the analyte was eluted with a small aliquot of methanol, which can be directly injected onto the assay system. Various factors were considered systematically. Parameters such as fiber diameter, packing amount of the naofibers, and pH of liquid phase were evaluated. The procedure was validated in terms of linearity (y=0. 999 4 for concentrations ranging from 10 to 5 000 ng/mL), precision (4. 4 and 1. 6%, respectively at 100 and 500 ng/mL), sensitivity (5 ng/mL for LOD) and extraction recovery (80. 6%-90. 2%). The developed procedure was found suitable for forensic investigations and was considered a good alternative to the liquid-liquid extraction methods normally used for the determination of this compound in biological media.