Evidence for the interaction of two optically coupled whole blood samples in the course of respiratory burst in one of them

摘要

Abstract: Possibility of interaction between the two optically coupled samples of whole human blood as studied. A quartz cuvette was placed inside a vial for a liquid scintillation counter. Saline diluted whole blood was poured into the vial and either undiluted blood or saline was poured into the cuvette. Respiratory burst (RB) was initiated in blood placed into the vial, and luminol-dependent chemiluminescence (LCL) was registered. Blood placed in the cuvette affected photon emission from blood placed in the vial. Blood of another group of donors enhanced photon emission from the 'partner' sample. Some properties of blood taken from the cuvette after being in the contact with the sample in which RB was induced changed in comparison with the same blood that was contacting with the non-stimulated sample. Exposed blood has lost the ability to attenuate light emission from the fresh portion of blood in which RB was induced. Besides its own LCL in response to addition of zymosan was different from that of the control These results suggest that two chemically separated but optically coupled samples of blood can interact. !17