Some ideas towards a grammatical model of the /spl sigma//sup 54/ bacterial promoters

摘要

The integration of large amounts of information on the regulation of gene expression requires conceptual frameworks which should facilitate the discovery of general principles underlying different mechanisms of gene regulation. An exhaustive database of /spl sigma//sup 70/ and /spl sigma//sup 54/ promoters in E. Coli has supported the construction of a grammatical model of the /spl sigma//sup 70/ type of promoters. This grammar generates all and only those regulatory arrangements of the collection, as well as new ones which are consistent with the biological principles of the collection. In this paper, some ideas towards a unified model of the /spl sigma//sup 70/ and the /spl sigma//sup 54/ bacterial promoters are presented. This model should reflect the biological differences on the possible regulatory mechanisms of these collections. The /spl sigma//sup 54/ class represents an intermediate step between the 7/sup 0/ promoters and those present in higher organisms. Based on the biology of these bacterial promoters a hypothesis is proposed which stipulates that in principle it is feasible to activate /spl sigma//sup 70/ promoters at a distance, an exclusive property of the /spl sigma//sup 54/ class shared with promoters from higher organisms. Assuming this hypothesis is correct, some a ideas supporting a unique "universal" grammar for the /spl sigma//sup 70/ and /spl sigma//sup 54/ promoters are presented.