A Dual-Channel Design in Microfluidic Chip for Label-Free Detection by Quasi-Bessel Beam

摘要

Scattering signals in microfluidic chips are widely used to detect cells by label-free method. However, it is hard to acquire the accurate scattering signals due to the different motion state when the cells passing through the detection area. In this paper, a dual-channel was designed to acquire the forward small-angle scattering signals which carries lots of biological information and quasi-Bessel beam was chosen as illumination source. Through the method of dual-channel acquisition, scattering signals in two angles can be detected simultaneously. This method has a great improvement in detection accuracy and the efficiency of obtaining biological information.