APPARATUS AND METHOD FOR LABEL-FREE SENSING BY ELECTROKINETIC STREAMING POTENTIAL IN MICROFLUIDIC-CHIP BASED PATHOGEN DETECTION

摘要

A new label-free sensing method and apparatus for detecting pathogens from changes in the interfacial electrical flow stream potential based on a microfluidic chip. The sensing method and apparatus are characterized in that a pathogen sample liquid is flowed through a microchannel filled with at least one glass bead with an antimicrobial peptide immobilized thereon and the pathogen is bound to the glass beads through the interaction with the antimicrobial peptide over time The sensing method and apparatus are based on the principle that the chargeability of the beads is changed and the flow potential is changed. The sensing method and apparatus shortens the total analysis time in detection of pathogens such as bacteria and viruses, secures detection stability, The field diagnosis function can be enhanced.