Determination of amines using HPLC with fluorescence detection

摘要

A simple and sensitive method for the determination of free aliphatic and aromatic amines using derivatives agents (dansyl chloride, 9-fluorenylmethyI chloroformate and 4-Chloro-7-nitrobenzofurazan) as a labelling reagent by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. A fluorescence derivatization - high-performance liquid chromatography (HPLC) method, which enables the femtomole-level detection of analytes. is a powerful tool for the analysis with high sensitivity and selectivity. In recent decades, improvised explosive devices (lEDs) have become an increasing topic of public concern, with high-profile incidents such as the Bali (2002). London transport (2005). Boston Marathon (2013) and Brussels (2016) bombings garnering mass international attention./1 ] Although the majority of media coverage has focused on large-scale incidents, there has also been a rise in explosive incidents involving smaller devices such as pipe bombs. This is particularly the case in the United States, where pipe bombs are considered to make up the vast majority of IED encounter. These devices can be easily constructed from everyday materials.[2] The mixture nitromethane with amines is applicable for this. Determination of residues after an explosion is important. This method (HPLC-FLD) could be useful for their determination. The proposed method is sensitive and reproducible for the determination of aliphatic and aromatic amines.