Quantifying Cell-Fibronectin Adhesion Forces by AFM Single-Cell Force Spectroscopy

摘要

Cell to cell interactions can lead to cancer cancerous cells migration and invasion. Atomic force microscopy (AFM) provides a tool that can study the physical properties of nanoscale spatial resolution cells. By attaching individual cells onto the cantilever of AFM, cellular adhesion forces can be quantified, and this technique is called single-cell force spectroscopy (SCFS). In this work, we used AFM-based SCFS to detect adhesion forces between different cells (including cancerous cells and normal cells) and fibronectin, which improves our understanding of cellular adhesion behaviors.